Abstract
In order to create a system of seed production of potatoes in the short of period, it is necessary to improve the formation of in vitro microtubers. The formation of microtubers when 5 mg / l kinetin was added to the MS feed showed the best performance. Normal MS feed did not produce microtubers in vitro. The formation of microtubers in the dark state was better than in the light state. The combination of MS + 8% sucrose + 5 mg / l kinetin was the best indicator for the formation of in vitro microtubers studied.
References
Abbott AJ and Belcher AR (1986) Potato tuber formation in vitro. In: Plant Tissue Culture and its Agricultural Applications. Withers LA and Alderson PG (eds.) Butterworths. London. pp 113-122
Al-Momani F, Shibli R and Ajloum M (2000) In vitro performance of potato (Solanum tuberosum L.) cv Spunta. J Agrotrop. 11: 31-4
Amma K and Maity S (1998) Role of nodal position and hormones on microtuber production in potato (Solanum tuberosum L.). J Hort. 11: 65- 7
Coleman KW, Danielle JD and Colleman SE (2001) Potato microtuber as research tools: A Review. Am J Potato Res. 78: 47-55
El-Sawy A, Bekheet S and Aly UI (2007) Morphological and molecular characterization of potato microtubers production on coumarin iducing medium. In J Agri Biol. 9(5): 675-680 http://www.fspublishers.org
Gopal J, Minocha JL and Dhaliwal HS (1998) Microtuberization in potato (Solanum tuberosum L.) Pl Cell Rep. 17: 794-8
Hoque MI (1996) In vitro microtuberization. Bangladesh J Bot. 25: 87-93
Hossain MJ and Sultana N (1998) Effect of Benzyl Amino Purine (BAP) and Chloro Choline Chloride (CCC) on in vitro tuberization of potato. Bangladesh J Ag Res. 23(4): 685-690.
Hussey G and Stacey NJ (1981) In vitro propagation of potato (Solanum tuberosum L.) Ann Bot. (London) 48: 787-796
Hussey G and Stacey NJ (1984) Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.) Ann Bot. 53: 565-578
Kanwal A, Ali A and Shoaib K (2006) In vitro microtuberization of potato (Solanum tuberosum L.) cultivar kuroda- A new variety in Pakistan. Int J Agri. 8(3): 337-340
Kotkas K and Peter K (1998) Preservation techniques of potato genetic resources in vitro Potato Res. 4: 94-103
Leclerc Y, Danielle J, Donnelly K and Seabrook JEA (1994) Microtuberization of layered shoots and nodal cuttings of potato: the influence of growth regulators and incubation periods. Pl Cell Tiss Org Cult. 37: 113-20
Mares DJ, Marschner H and Kruss A (1981) Effect of gibberellic acid on growth and carbohydrate metabolism of developing tuber of potato (Solanum tuberosum L.) Physiol Plant. 52: 267-274
Myeong C, Yiens, Park YE, Kim KJ, Cho HM and Hann HB (1990) Study on seed potato influences of several factors on in vitro tuberization of shoot nodes in potato c.v. Dejima. Res. Dep. Rural. Dev. Adm (Shweon). 3: 46-53
Pelacho Am, Closas ML, Campabadal C, Torres A, Farran I and Mingo-Castel AM (1994) In vitro tuberization of potato: Effect of several morphogenic regulators in light and darkeness J Pl Physiol. 144: 705-9
Shibbi R, Abu-Ein AM and Ajlouni M (2001) In vitro and in vitro multiplication of virus free "Spunta" potato. Pakistan J Bot. 33: 35-41
Tovar PR, Estrada L, Schilde-Rentschler and Dodds JH (1985) Induction and use of in vitro potato tubers. CIP Circular, International Potato Center 13: 1-4
Tugrul S and Samanci B (2001) Factors affecting tuber formation in potato (Solanum tuberosum L.) Potato Abstr. 26: 86
Vreugdenhil D and Struik PC (1989) An Integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum L.) Physiol Pl. 75: 525-31